Journal: Oncology Letters
Article Title: Role of BCL2-associated athanogene in resistance to platinum-based chemotherapy in non-small-cell lung cancer
doi: 10.3892/ol.2015.4003
Figure Lengend Snippet: Tunicamycin-induced endoplasmic reticulum stress in A549 cells. (A) Effects of tunicamycin on the growth of A549 cells. Growth inhibition of A549 cells was examined using MTT assay. Data are presented as the mean ± standard deviation from experiments conducted in triplicate. (B) Impact of tunicamycin on GRP78 and BAG1. A549 cells were treated with 1.25 µg/ml of tunicamycin for 8 h, followed by immunoblot using the indicated antibodies. GRP78, glucose regulated protein 78; BAG1, BCL2-associated athanogene; L, p50 isoform; M, p46 isoform; S, p33 isoform.
Article Snippet: A polyclonal rabbit anti-mouse antibody against 3 isoforms of BAG1 [p50 (BAG1L), p46 (BAG1M) and p33 (BAG1S)] (cat. no. sc-939) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).
Techniques: Inhibition, MTT Assay, Standard Deviation, Western Blot